Freund's complete adjuvant (FCA)

This assay uses certain amount of CFA, which is injected into hind paw of rodents. Several days after CFA injection, the paw pressure threshold is measure by using Randall – Selitto clip and compared with baseline (the pressure threshold before injection of CFA). The decrease of threshold indicates that the hyperalgesia is induced by CFA and effective analgesics are able to bring back or reverse the threshold to baseline or above.